| Gene Expression Analysis | ||
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Using cDNA microarrays,
researchers are able to identify entire sets of differentially
expressed genes under defined conditions. Those comparative expression data need to be validated by additional approaches to confirm that the hits identified were indeed differentially expressed. |
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Quantitative
Real-Time PCR  |
4base lab specializes in
developing custom fluorescence-based PCR assays using the LightCycler
technology to confirm expression data derived from microarray analysis.
This type of analysis is specific for particular RNA targets within a given mRNA population. The target transcript will be quantified in relation to constitutive expressed reference genes of the sample. |
Melting Curve
Analysis
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The development of a specific qPCR assay includes
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