Custom Mutagenesis
 
4base lab offers this service to make small changes within your cloned DNA in order to correct for PCR artifacts or to substitute particular amino acids.
Any additional secondary alteration of the sequence is almost eliminated by our procedure. This gives you the confidence working with a correct clone when using the rDNA in extensive downstream experiments.

  • simple
  • reliable
  • fast
  • low cost
The standard service includes
  • modification of one position
  • verification of the altered site by DNA sequencing
  • no set-up charge in case of failure

we need
  • sequence of your insert via e-mail
  • position and type of modification seeked
  • recombinant S1 bacteria or 10 µg dsDNA
    (type of vector/resistance marker)
 you get
  • transformed E. coli clone
  • sequence chromatogram verifying the modification