Transcript 5' Mapping
 
To pinpoint the 5' end of mRNA greatly facilitates the understanding of the architecture and regulation of gene loci. It allows to map the appropriate promoter(s) from which transcription is initiated and other control or recognition elements upstream of the target gene.

4base lab utilizes a fluorescence based system and established protocols in order to determine the transcriptional start of your mRNA.
Please contact our staff to discuss further details.

 
 
FIG: Comparative analysis of initiation points of mRNA derived from the wild type (blue) and a mutant clone (green). The region shown encompasses a promoter array.